1) Task 1, Project Management and Administration (Ongoing)

2) Task 2, Assemble a Technical Advisory Committee (Ongoing)

3) Task 3, Bacteriological Survey of Watershed and Adjacent Beach Recreational Water. "Bacteriological Survey of San Juan Creek Watershed" report, which contains methods, sampling plan, results and conclusions, is attached.

Study Tasks in Progress:

1) Task 4, Detailed Bacteriological Survey of Identified Problem Areas -
Sampling started September 18th. The sampling plan consists of focusing on five sites:

1) SJ10, Trabuco Creek below Ramos St.
2) SJ6 San Juan Creek above Trabuco Creek
3) SJC2 San Juan Creek at PCH
4) SJ02, San Juan Creek at the beach (behind berm)
5) SJ25, Ocean at San Juan Creek

This plan will allow the subsequent source-tracking portion of the study to compare the source species of bacterial pollution upstream at two different creeks vs. the creek mouth at the beach vs. the ocean water. This plan was discussed with the regional board representative Jeremy Haas on Aug 10. Total coliform, fecal coliform, enterococcus and E. coli bacteria levels are being quantitated once a week from each site for 10 weeks. The sampling will be complete November 19.

2) Task 5, Source Identification by ARA and Ribotyping
Sampling started September 18th in conjunction with Task 4. Environmental sample collection will be complete November 19. Approximately 50% of known species isolates from human, sewage, cat, dog, horse and seagull have been collected and processed. Isolates are being forwarded to the three collaborators for ARA analysis (Dr. Harwood) and Ribotyping (Dr. Lukasik and Dr. Samadpour). Isolation and transportation of known isolates to collaborators should be complete in December.

Contract Activities:

1) MOU between Orange County PFRD and HCA Public Health Laboratory is in the process of being finalized.

TAC Meetings:

None held.